Question.4328 - Blood Typing Activity Assignment InstructionsOverviewBlood groups are genetically determined, and not influenced by environmental factors. Blood typing is conducted to determine a person’s blood group. In this investigation, students are introduced to the ABO and Rh blood typing systems, the process of agglutination, and the use of agglutination in the typing of human blood. Students carry out a blood typing procedure using simulated (synthetic) blood and antisera. InstructionsRead the PDF on Lab Safety and the Blood Typing with Simulated Blood Investigation Manual in the Learn section of the course before beginning this assignment. Preparatory Steps: Put on PPE: gloves, lab apron, and goggles. On a sheet of white paper, clearly handwrite labels as follows: **Sample #1: Your full name. Repeat this on separate sheets of paper for all 4 samples (changing sample # each time). Have a digital/phone camera ready to take pictures of the results of each sample. Experimental Steps: Using the dropper vial, place a drop of the first simulated blood sample in each well of the blood typing slide. Replace the cap on the dropper vial. Add a drop of simulated anti-A serum (blue) to the well labeled A (Making sure you don’t touch the tip to the blood sample). Replace the cap. Add a drop of simulated anti-B serum (yellow) to the well labeled B. Replace the cap. Add a drop of simulated anti-Rh serum (clear) to the well labeled Rh. Replace the cap. Using a different color-mixing stick for each well (blue for anti-A, yellow for antiB, white for anti-Rh), gently stir the simulated blood and antiserum drops for 30 seconds. Make sure to stir for the full 30 seconds or your results may be inaccurate! Carefully examine the thin films of liquid mixture that are left behind. If a film remains uniform in appearance, there is no agglutination. If the sample appears granular, agglutination has occurred and record it as + in the table below. No agglutination should be represented by a -. Determine the blood type of the sample using the table below as a guide. Establish whether or not agglutination has occurred in each sample. A positive agglutination reaction indicates the blood type. Anti- A Vial Anti-B Vial Anti-Rh Vial Blood Type Sample 1 Sample 2 Sample 3 Sample 4 *This table will NOT be submitted as is, it is simply for you to record your observations to complete the final submission product. Document Results: Set the slide on the white piece of paper that you labeled earlier. Make sure your photo includes the labels you wrote as well as the whole slide. **Ensure you are creating a quality photo with good lighting. The instructor needs to be able to distinguish is agglutination is taking place in the picture. Repeat steps 1-8 with the remaining blood samples. Disposal and Cleanup Dispose of solutions down the drain with the water running. Allow the faucet to run a few minutes to dilute the solutions. Rinse and dry the lab, equipment and return the materials to your equipment kit. All other investigation materials may be disposed of as household trash. Sanitize the workspace. Presenting Results Download Blood Typing Activity Submission Template PowerPoint file. Type in your name on Title page. Fill in the slides appropriately. Each slide requires you to fill in the table for the particular sample as well as a picture of your results for the sample. See figure below: Save your file as a PowerPoint with the following format: Blood Typing Activity_lastname, first name Upload your results as a PowerPoint file to the Assignment link.

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